Coding

Part:BBa_K3221204:Design

Designed by: Yaxin Luo   Group: iGEM19_SCU-China   (2019-10-04)


Reporter sequence of delay system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
    Illegal XhoI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3560
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
    Illegal BsaI site found at 637
    Illegal BsaI.rc site found at 2313
    Illegal BsaI.rc site found at 4609
    Illegal SapI site found at 1002


Design Notes

We add a non-coding sequence before the GAL4 sequence to avoid transcoding mutation.


Source

The original sequence comes from part BBa_K3221203 and BBa_K3221200.

References